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. 2009 Sep 17;284(46):31616–31624. doi: 10.1074/jbc.M109.025882

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Mitochondrial PARP-1 is associated to mitochondrial DNA. A, PCR analysis after chromatin immunoprecipitation with an antibody against PARP-1 showed the amplification of the desired product size in a different region of the mtDNA. DNA sample lane descriptions are as follows. Input, samples isolated from total lysates prior to antibody pulldown as an internal positive control; No Ab, samples isolated after pull down with no antibody; PARP-1, samples isolated after PARP-1 pull down; H₂O, PCR negative control. B, HeLa cells were transfected with Mitofilin siRNA or control siRNA and analyzed as in A. The densitometric analysis of band intensity (ImageJ software) indicated that immunoprecipitation of Mitofilin-specific siRNA (MITO-siRNA)/nonspecific (NS-siRNA) siRNA = 0.5.