FIGURE 2.

In vitro import of pSu9-DHFR and pX into WT and tom70Δ mitochondria. Radiolabeled pSu9-DHFR (A) or radiolabeled precursor proteins pAco1p, pAtp1p, pCit1p, pDld2p, pIdh1p, and pAtp2p (B) were incubated with wild type (WT, open circles) or tom70Δ (filled circles) mitochondria (0.1 mg of protein/ml) at 25 °C for indicated times. The mitochondria were treated with proteinase K and imported, protease-protected proteins were analyzed by SDS-PAGE and radioimaging. The amounts of radiolabeled proteins added to each reaction are set to 100%. p, precursor forms; m, mature forms; 10%, 10% of the input; −ΔΨ, valinomycin was added.