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. 2009 Sep 18;284(46):31635–31646. doi: 10.1074/jbc.M109.041756

FIGURE 3.

FIGURE 3.

In vitro import of pX-DHFR and pX(5)-DHFR into wild type and tom70Δ mitochondria. A, radiolabeled fusion proteins pAco1p-DHFR, pAtp1p-DHFR, pCit1p-DHFR, pDld2p-DHFR, pIdh1p-DHFR, and pAtp2p-DHFR were imported into wild type (WT) or tom70Δ mitochondria (0.1 mg of protein/ml) and analyzed as in Fig. 2. B, radiolabeled fusion proteins pAco1(5)-DHFR, pAtp1(5)-DHFR, pCit1(5)-DHFR, pDld2(5)-DHFR, pIdh1(5)-DHFR, and pAtp2(5)-DHFR were imported into wild type (WT) or tom70Δ mitochondria (0.1 mg of protein/ml) and analyzed as in Fig. 2. The amounts of radiolabeled proteins added to each reaction are set to 100%. p, precursor forms; m, mature forms; 10%, 10% of the input; −ΔΨ, valinomycin was added.