FIGURE 6.

In vitro import of mature parts of pX fused to a Tom70-independent presequence depends on Tom70. A, radiolabeled fusion proteins pCox4-Aco1p, pCox4-Atp1p, pCox4-Cit1p, pCox4-Dld2p, pCox4-Idh1p, and pCox4-Atp2p were imported into wild type (WT) or tom70Δ mitochondria (0.1 mg of protein/ml) and analyzed as in Fig. 2. B, radiolabeled fusion proteins pSu9-Aco1p, pSu9-Atp1p, pSu9-Cit1p, pSu9-Dld2p, and pSu9-Atp2p were imported into Tom70T mitochondria (0.1 mg of protein/ml) with or without TEVp treatment (right panels) and analyzed as described for Fig. 2. The amounts of radiolabeled proteins added to each reaction are set to 100%. p, precursor forms; m, mature forms. 10%, 10% of the input; −ΔΨ, valinomycin was added.