TABLE 1.
Plasmids used in this study
aa, amino acids.
| Plasmid | Vector | Relevant characteristics | Source or reference |
|---|---|---|---|
| pSu9-DHFR | pGEM-4Z | Presequence of Su9 (1–69 aa) and DHFR (187 aa) | Ref. 30 |
| pHsp60 | pGEM-4Z | Hsp60 precursor (1–572 aa) | This study |
| pCox4p | pGEM-4Z | Cox4 precursor (1–155 aa) | Ref. 28 |
| pAco1p | pGEM-4Z | Aco1 precursor (1–778 aa) | This study |
| pAtp1p | pGEM-4Z | Atp1 precursor (1–545 aa) | This study |
| pCit1p | pGEM-4Z | Cit1 precursor (1–479 aa) | This study |
| pDld2p | pGEM-4Z | Dld2 precursor (1–530 aa) | This study |
| pIdh1p | pGEM-4Z | Idh1 precursor (1–360 aa) | This study |
| pAtp2p | pGEM-4Z | Atp2 precursor (1–511 aa) | This study |
| pAco1p-DHFR | pGEM-4Z | Aco1 precursor (1–778 aa) and DHFR (187 aa) | This study |
| pAtp1p-DHFR | pGEM-4Z | Atp1 precursor (1–545 aa) and DHFR (187 aa) | This study |
| pCit1p-DHFR | pGEM-4Z | Cit1 precursor (1–479 aa) and DHFR (187 aa) | This study |
| pDld2p-DHFR | pGEM-4Z | Dld2 precursor (1–530 aa) and DHFR (187 aa) | This study |
| pIdh1p-DHFR | pGEM-4Z | Idh1 precursor (1–360 aa) and DHFR (187 aa) | This study |
| pAtp2p-DHFR | pGEM-4Z | Atp2 precursor (1–511 aa) and DHFR (187 aa) | This study |
| pAco1(5)-DHFR | pGEM-4Z | Presequence of Aco1 (1–28 aa) and DHFR (187 aa) | This study |
| pAtp1(5)-DHFR | pGEM-4Z | Presequence of Atp1 (1–47 aa) and DHFR (187 aa) | This study |
| pCit1(5)-DHFR | pGEM-4Z | Presequence of Cit1 (1–42 aa) and DHFR (187 aa) | This study |
| pDld2(5)-DHFR | pGEM-4Z | Presequence of Dld2 (1–49 aa) and DHFR (187 aa) | This study |
| pIdh1(5)-DHFR | pGEM-4Z | Presequence of Idh1 (1–34 aa) and DHFR (187 aa) | This study |
| pAtp2(5)-DHFR | pGEM-4Z | Presequence of Atp2 (1–37 aa) and DHFR (187 aa) | This study |
| pAco1(5)-Hsp60 | pGEM-4Z | Presequence of Aco1 (1–28 aa) and mature part of Hsp60 (22–572 aa) | This study |
| pAtp1(5)-Hsp60 | pGEM-4Z | Presequence of Atp1 (1–47 aa) and mature part of Hsp60 (22–572 aa) | This study |
| pCit1(5)-Hsp60 | pGEM-4Z | Presequence of Cit1 (1–42 aa) and mature part of Hsp60 (22–572 aa) | This study |
| pDld2(5)-Hsp60 | pGEM-4Z | Presequence of Dld2 (1–49 aa) and mature part of Hsp60 (22–572 aa) | This study |
| pIdh1(5)-Hsp60 | pGEM-4Z | Presequence of Idh1 (1–34 aa) and mature part of Hsp60 (22–572 aa) | This study |
| pAtp2(5)-Hsp60 | pGEM-4Z | Presequence of Atp2 (1–37 aa) and mature part of Hsp60 (22–572 aa) | This study |
| pAco1(5)-Cox4p | pGEM-4Z | Presequence of Aco1 (1–28 aa) and mature part of Cox4 (26–155 aa) | This study |
| pAtp1(5)-Cox4p | pGEM-4Z | Presequence of Atp1 (1–47 aa) and mature part of Cox4 (26–155 aa) | This study |
| pCit1(5)-Cox4p | pGEM-4Z | Presequence of Cit1 (1–42 aa) and mature part of Cox4 (26–155 aa) | This study |
| pDld2(5)-Cox4p | pGEM-4Z | Presequence of Dld2 (1–49 aa) and mature part of Cox4 (26–155 aa) | This study |
| pIdh1(5)-Cox4p | pGEM-4Z | Presequence of Idh1 (1–34 aa) and mature part of Cox4 (26–155 aa) | This study |
| pAtp2(5)-Cox4p | pGEM-4Z | Presequence of Atp2 (1–37 aa) and mature part of Cox4 (26–155 aa) | This study |
| pCox4-Aco1p | pGEM-4Z | Presequence of Cox4 (1–25 aa) and mature part of Aco1 (24–778 aa) | This study |
| pCox4-Atp1p | pGEM-4Z | Presequence of Cox4 (1–25 aa) and mature part of Atp1 (43–545 aa) | This study |
| pCox4-Cit1p | pGEM-4Z | Presequence of Cox4 (1–25 aa) and mature part of Cit1 (38–479 aa) | This study |
| pCox4-Dld2p | pGEM-4Z | Presequence of Cox4 (1–25 aa) and mature part of Dld2 (45–530 aa) | This study |
| pCox4-Idh1p | pGEM-4Z | Presequence of Cox4 (1–25 aa) and mature part of Idh1 (30–360 aa) | This study |
| pCox4-Atp2p | pGEM-4Z | Presequence of Cox4 (1–25 aa) and mature part of Atp2 (33–511 aa) | This study |
| pSu9-Aco1p | pGEM-4Z | Presequence of Su9 (1–69 aa) and mature part of Aco1 (24–778 aa) | This study |
| pSu9-Atp1p | pGEM-4Z | Presequence of Su9 (1–69 aa) and mature part of Atp1 (43–545 aa) | This study |
| pSu9-Cit1p | pGEM-4Z | Presequence of Su9 (1–69 aa) and mature part of Cit1 (38–479 aa) | This study |
| pSu9-Dld2p | pGEM-4Z | Presequence of Su9 (1–69 aa) and mature part of Dld2 (45–530 aa) | This study |
| pSu9-Idh1p | pGEM-4Z | Presequence of Su9 (1–69 aa) and mature part of Idh1 (30–360 aa) | This study |
| pSu9-Atp2p | pGEM-4Z | Presequence of Su9 (1–69 aa) and mature part of Atp2 (33–511 aa) | This study |
| pRS314/Tom70(WT) | pRS314 | Expression of wild type Tom70 under the control of own promoter | This study |
| pRS314/Tom70(38TEV) | pRS314 | Expression of Tom70(38TEV); TEV protease recognition sequence was inserted between residues 38 and 39 | This study |
| pRS314/Tom70(98TEV) | pRS314 | Expression of Tom70(98TEV); TEV protease recognition sequence was inserted between residues 98 and 99 | This study |
| pRS314/Tom70(246TEV) | pRS314 | Expression of Tom70(246TEV); TEV protease recognition sequence was inserted between residues 246 and 247 | This study |
| pRS314/Tom20(WT) | pRS314 | Expression of wild type Tom20 under the control of own promoter | Ref. 10 |
| pRS314/Tom20(73TEV) | pRS314 | Expression of Tom20(73TEV); TEV protease recognition sequence was inserted between residues 73 and 74 | Ref. 10 |