FIGURE 1.

8-oxoG directs slow adenine misincorporation. a, base pairing properties of 8-oxoG. b, nucleic acid scaffolds (color coding used throughout). RNA was 5′-end-labeled with 6-carboxyfluorescein and a UGCAU linker. Filled circles denote nucleotides present in the structures (see Fig. 3). c, RNA extension with EC A. d, time courses of incorporation with CTP or misincorporation with ATP. The indicated complementary scaffold was used. The DNA non-template was 5′-end-labeled with biotin using a TTTTT linker. The RNA was 5′-end-labeled with 6-carboxyfluorescein and a UGCAU linker.