FIGURE 6.

Overexpression of PDI does not affect the levels of catalytically active AChE monomers and dimers. HEK 293 stable cells expressing chAChE were transfected with expression vector encoding chPDI (open circles). The control consisted of transfection with an empty vector alone (solid circles). The different AChE forms expressed in total cell extracts were fractionated by velocity sedimentation, and their enzymatic activity was measured. G1 and G2 refer to AChE monomers and dimers. The arrow indicates most likely AChE tetramers.