TABLE 1.
Weight, plasma, and liver chemistries of mice fed the control, MCD, MCD + homocysteine, or homocysteine diets for 14 days
Values are expressed as mean ± S.D. n = 7 for all parameters except plasma homocysteine for which n = 5 pooled samples with 2–3 mice per sample. The asterisk indicates that in a subgroup of mice in which homocysteine-infused drinking water was replaced with distilled water for the 4-h fast prior to sacrifice, plasma homocysteine was 75.0 ± 3.8 μm in the MCD + homocysteine cohort and 29.0 ± 6.2 μm in the homocysteine cohort.
| Control | MCD | MCD + homocysteine | Homocysteine | |
|---|---|---|---|---|
| Plasma homocysteine (μm) | 13.6 ± 1.6 | 102.7 ± 17.2a | 113.0* ± 22.0a | 43.1* ± 7.8a,b |
| Body weight (g) | 21.0 ± 0.4 | 17.5 ± 1.1a | 21.1 ± 0.6b | 21.3 ± 2.2b |
| Change in body weight (%) | +1.6 ± 1.6 | −14.9 ± 1.4a | +3.5 ± 4.1b | +7.1 ± 7.8b |
| Plasma ALT (IU/L) | 35 ± 7 | 203 ± 57a | 129 ± 30a,b | 44 ± 9b |
| Plasma cholesterol (mg/dL) | 212 ± 17 | 102 ± 9a | 138 ± 8a,b | 192 ± 24b |
| Plasma triglyceride (mg/dL) | 241 ± 10 | 204 ± 28 | 206 ± 22 | 205 ± 27 |
| Plasma glucose (mg/dL) | 143 ± 32 | 79 ± 25a | 181 ± 17b | 109 ± 21b |
| Liver weight (g) | 1.00 ± 0.09 | 1.13 ± 0.16 | 1.42 ± 0.20a,b | 0.99 ± 0.06 |
| Liver wt/body wt (%) | 4.4 ± 0.2 | 6.5 ± 0.7a | 6.7 ± 0.8a | 4.5 ± 0.3b |
| Hepatic triglyceride (mg/mg protein) | 0.22 ± 0.06 | 0.72 ± 0.14a | 0.64 ± 0.12a | 0.23 ± 0.02b |
| Hepatic cholesterol (μg/mg protein) | 25.5 ± 3.2 | 47.6 ± 7.6a | 33.2 ± 8.3a,b | 26.6 ± 2.0b |
a p < 0.05 versus control.
b p < 0.05 versus MCD.