TABLE 3.
Phenotype and hepatic gene expression in mice fed the control or homocysteine diets for 6 weeks
Values are expressed as mean ± S.D. n = 6 for all parameters except plasma homocysteine for which n = 3 pooled samples with 2 mice per sample.
| Control | Homocysteine | |
|---|---|---|
| Plasma homocysteine (μm) | 21.0 ± 3.2 | 134.7 ± 29.5a |
| Body weight (g) | 20.5 ± 1.7 | 24.4 ± 1.3a |
| Change in body weight (%) | +1.8 ± 3.3 | +15.1 ± 3.3a |
| Plasma ALT (IU/L) | 27 ± 5 | 42 ± 10 |
| Liver weight (g) | 1.00 ± 0.05 | 1.07 ± 0.11 |
| Liver wt/body wt (%) | 4.9 ± 0.5 | 4.4 ± 0.4 |
| Hepatic triglyceride (mg/mg protein) | 0.33 ± 0.11 | 0.41 ± 0.08 |
| Hepatic SAM (nmol/g liver) | 52.4 ± 6.4 | 63.6 ± 5.8 |
| Hepatic SAH (nmol/g liver) | 69.7 ± 6.0 | 74.9 ± 18.2 |
| SAM/SAH ratio | 0.8 ± 0.1 | 0.9 ± 0.2 |
| Relative mRNA levels | ||
| BiP | 1.05 ± 0.34 | 0.37 ± 0.11a |
| CHOP | 1.03 ± 0.32 | 1.02 ± 0.13 |
| XBP-1(s) | 1.35 ± 1.20 | 0.27 ± 0.15a |
a p < 0.05 versus control.