FIGURE 4.

IL-25 up-regulates the expression of TJ proteins, occludin, JAM, and claudin-5. MBEC4 cells were incubated with or without graded doses of IL-25 alone (A) or with or without graded doses of IL-25 plus TNF-α (C) for 24 h. The expression of occludin, JAM, and claudin-5 in whole cell lysates was analyzed by Western blotting. The results of A and C are expressed graphically in B and D, respectively. The expression levels in untreated cells were set to 1. Data are represented as the means ± S.E. *, p < 0.05; n = 4. NT, not treated.