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. 2009 Sep 23;284(46):31882–31892. doi: 10.1074/jbc.M109.014522

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Mass spectra of the reaction products of BacA and BacB. a, ESI spectra of BacA incubated with prephenate in the positive ionization mode. b, ESI spectra of the product formed by the incubation of BacA and BacB with prephenate in positive ionization mode. c, ESI spectra of the product formed by the incubation of BacA and BacB with prephenate in and in negative ionization mode. The mass of barium prephenate is 361.49 Da. The deconvoluted mass obtained by ESI-MS is 226.9 Da in the positive ion mode, which corresponds to the mass of prephenate without barium (calculated mass 226.04 Da). Incubation of BacA with prephenate results in products with masses of 181.9, 182.9, and 183.9 Da in the positive ionization mode (isotopic splitting). The difference in mass between the substrate and the product (45 Da) confirms the decarboxylation reaction catalyzed by BacA. The incubation of BacA and BacB with prephenate results in products with masses 182.9 (183.9 Da) in the positive ion mode and 180.9 (181.9 Da) in the negative ionization mode (with isotopic splitting).