Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 17;284(46):31962–31971. doi: 10.1074/jbc.M109.022657

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Screen of putative lipid ligands for the two TaPEAMT isoforms. A, membrane lipid strips spotted with 100 pmol of each lipid were incubated with 80 nm recombinant TaPEAMT protein as before. As expected both isoforms strongly interact with PA, followed by some binding to PtdIns(4)p > PtdIns(4,5)P₂/cardiolipin. Numbers next to each chemiluminescent signal are given as percent spot intensity of PA binding to TaPEAMT1. B, to test the specificity of the interaction with phosphoinositides PIP strips (spots contain 100 pmol of lipid each) were incubated with 80 nm recombinant TaPEAMT protein as before. The strongest interaction again is observed with PA, followed by interactions with the three PtdIns monophosphates ((5)P > (4)P > (3)P) and two of the PtdIns diphosphates ((3,4)P₂ ≥ (4,5)P₂) consistent with earlier findings, except that TaPEAMT2 binding to PtdIns(4,5)P₂ was not detected in A most likely due to a more stringent wash. The recognition appears to be specific, because no binding to PtdIns, PtdIns(3,5)P₂, or PtdIns(3,4,5)P₃ was observed. Numbers next to each chemiluminescent signal describe spot intensities expressed as percentage of spot intensity for PA binding to TaPEAMT1.