Figure 4.

Hybridization of the TR-1 element to a blot panel of maize genomic DNA (Seneca 60) digested with different restriction enzymes. A typical ladder-like pattern of bands hybridizing to TR-1 is seen in lanes with DNA cut by EcoRI, AluI, RsaI, and Sau3A, the restriction enzymes that have a recognition site within TR-1. DNA samples cut with HaeIII, TaqI, and NdeI, which have no recognition sites within TR-1, reveal maximum hybridization signal with DNA fragments larger than 10–20 kb. StyI produced an intermediate ladder-like pattern of hybridization.