Skip to main content
. Author manuscript; available in PMC: 2010 Dec 16.
Published in final edited form as: J Am Chem Soc. 2009 Dec 16;131(49):17742–17743. doi: 10.1021/ja908378y

Table 1.

Kinetic Parameters for the AAG-catalyzed hydrolysis of Inosine in Different Structural Contextsa

Opposing Base K1/2 (μM) kmax (min−1) kmax/K1/2b (M−1s−1) Relative kmax/K1/2
Bulge (none) 0.08 5.4 1.1×106 3

T 0.30 6.3 3.5×105 (1)
G 0.41 1.5 6.2×104 0.2
C 3.8 3.3 1.4×104 0.04
A 2.3 2.0 1.4×104 0.04

Single strand 0.34 0.0035 1.7×102 0.0005
a

Data from 23 °C in 50 mM NaMES, pH 6.1, 1 mM EDTA, 1 mM DTT, 0.1 mg/mL BSA. Ionic strength was adjusted to 200 mM with NaCl.

b

kmax/K1/2 is a measure of the catalytic efficiency, analogous to kcat/KM in steady state kinetics.