Table 2. T1ρ Dispersion Analysis of Crosslinked BSA and Tissues.
γB1 Values (kHz) | Model Curve Fit (% Standard Error of the Estimate) | Relaxation-Time Power Law (Eq. [1]) Parameters of Fit | ||||||||
Sample Groups | Samples | Number of values | Minimum | Maximum | Relaxation-Time Power Law Eq. [1] | Relaxation-Rate Power Law Eq. [2] | BPP Model Eq. [3] | “a” (ms) | “b” (10−6×sec1+C) | “c” (exponent) |
BSA (Fraction V) in H2O (2T) | 10% BSA, 80mM GA | 13 | 0.97 | 60.0 | 2.4 | 6.8 | 2.9 | 138 | 764 | 0.515 |
10% BSA, 100mM GA | 13 | 1.01 | 59.2 | 2.1 | 5.3 | 5.0 | 93.8 | 2247 | 0.425 | |
20% BSA, 200mM GA | 22 | 0.14 | 33.1 | 2.3 | 12 | 3.3 | 45.7 | 31.1 | 0.731 | |
BSA (Monomers) in H2O (2T) | 10% BSA, 60mM GA | 13 | 1.50 | 33.1 | 0.4 | 9.7 | 0.9 | 293 | 3.10 | 0.993 |
10% BSA, 80mM GA | 13 | 1.50 | 33.1 | 0.8 | 17 | 1.5 | 187 | 21.7 | 0.833 | |
10% BSA, 100mM GA | 13 | 1.50 | 33.1 | 0.9 | 4.0 | 1.9 | 133 | 298 | 0.596 | |
BSA (Fraction V) in H2O (4.7T) | 10% BSA, 60mM GA | 23 | 1.05 | 154 | 1.2 | 10 | 7.3 | 155 | 334 | 0.591 |
10% BSA, 80mM GA | 19 | 1.50 | 100 | 2.0 | 4.7 | 4.5 | 81.8 | 2464 | 0.411 | |
10% BSA, 200mM GA | 23 | 1.00 | 154 | 3.0 | 9.4 | 9.5 | 64.2 | 785 | 0.501 | |
10% BSA (Fraction V), 60mM GA (2T) | undialyzed | 23 | 0.10 | 33.1 | 1.2 | 8.2 | 1.9 | 156 | 42.7 | 0.757 |
dialyzed in excess DMSO | 23 | 0.10 | 33.1 | 2.7 | 18 | 1.8 | 53.2 | 3.98 | 0.964 | |
dialyzed in H2O | 15 | 0.79 | 22.8 | 1.4 | 4.8 | 2.8 | 91.9 | 466 | 0.577 | |
4.7T | rabbit myocardial tissue | 19 | 0.59 | 47.6 | 2.0 | 7.2 | 8.4 | 74.5 | 398 | 0.569 |
2T | rat myocardial tissue | 22 | 0.10 | 28.5 | 1.2 | 12 | 3.4 | 68.4 | 26.9 | 0.786 |
Cartilage (2T) | in saline | 23 | 0.21 | 46.0 | 1.7 | 6.0 | 2.9 | 87.4 | 509 | 0.484 |
in DMSO | 24 | 0.21 | 56.0 | 3.6 | 17 | 3.9 | 9.06 | 5.87 | 0.756 | |
in phosphate buffer pH 9.2 | 21 | 0.20 | 33.1 | 3.9 | 17 | 4.2 | 26.9 | 25.8 | 0.769 | |
in phosphate buffer pH 7.0 | 23 | 0.10 | 33.1 | 3.3 | 13 | 4.1 | 51.0 | 224 | 0.591 | |
in phosphate buffer pH 4.35 | 23 | 0.10 | 33.1 | 4.1 | 16 | 4.7 | 39.6 | 133 | 0.635 |
BSA = Bovine serum albumin, GA = Glutaraldehyde, DMSO = Dimethyl sulfoxide.