Figure 3.

Maps for the V_H1 and V_H4 segments on chromosomes 14 and 15. The alignment of the maps for chromosome 14 is read from left to right and then from top to bottom, and is subdivided into four portions by vertical bars (see text). Loci in the dashed-lined boxes are aligned putatively because sequence data in the map by Cook et al. (8) are not available. The superscript letters indicate the numbers of base pairs different between the allelic sequences within the amplified regions. The unresolved pairs, 4–9 and 4–1 in both haplotypes 4 and 7, 1–17 and 1–14, and 1–16 and 1–4 in haplotype 4 are placed in the alignment according to the order information in the map by Cook et al. (8). 1–9b and 1–1 in haplotype 7 could not be amplified by the family-specific primers and were placed on the map by analyzing additional sperm with both gene segment-specific and family-specific primers. V_H segments that are closely located on chromosome 15 are boxed. The CEs on the right of the maps may be oriented differently as indicated by the observation that segments 1–23 and 1–5 were codetected with 1–10 and 4–2 from a sperm with haplotype 2 but with 1–19 and 1–9 from a sperm with haplotype 3. The sequences with no counterpart sequences in the maps and the accession numbers of their counterpart sequences in the GenBank are: 1–3, Z12305 (10); 1–5, Z29632 (5); 1–8, Z29632 (5); 1–9, Z17390 (5); 1–10, Z29596 (5); 1–12, Z12312 (10); 1–12b, AF030491; 1–19, Z29631 (5); 1–20, AF030492; 1–22, Z29633 (5); 1–23, L25542 (6); 4–2, X92231 (52); and 4–3, U23548.