Table 4.
Control of R. annulatus infestations in cattle vaccinated with the recombinant antigens
| R. annulatus (Mission, TX strain) | |||||
|---|---|---|---|---|---|
| Percent reduction (vaccinated/control)b | Ec | ||||
| Experimental groupa | DT | DW | DO | DF | |
| Ubiquitin | −2% | −19% | −21% | 15% | 15% |
| (519 ± 96) | (275 ± 29) | (109 ± 15) | (0.13 ± 0.03) | ||
| Subolesin | 18% | 17% | 23% | 37% | 60% |
| (419 ± 288) | (192 ± 129) | (69 ± 49) | (0.09 ± 0.07) | ||
| Bm86 | 100% | All ticks | All ticks | All ticks | 100% |
| (0 ± 0)* | Died | Died | Died | ||
| Adjuvant/saline control | (509 ± 145) | (231 ± 28) | (90 ± 20) | (0.15 ± 0.03) | – |
DT percentage reduction in tick infestation, DW percentage reduction in tick weight, DO percentage reduction in oviposition, DF percentage reduction in egg fertility
aCattle were randomly assigned to experimental groups (N = 4), vaccinated, and challenged with R. microplus and R. annulatus larvae
bThe percent reduction was calculated with respect to the control group. In parenthesis are shown the average ± SD for adult female tick number, tick weight (milligram), oviposition (egg weight (milligram) per tick) and egg fertility (larvae weight per egg weight) and were compared by Student's t test with unequal variance between vaccinated and control groups (*P < 0.05)
cVaccine efficacy (E) was calculated as 
, where CRT, CRO, and CRF are the reduction in the number of adult female ticks, oviposition, and egg fertility as compared to the control group, respectively