Fig. 8.
Purification of the heterologously expressed GAPN from S. solfataricus by using the E. coli pET expression system. HP Heat precipitation at 70°C, IEC ion exchange chromatography, GF gelfiltration, M protein ladder (Page ruler™, fermentas)
Purification of the heterologously expressed GAPN from S. solfataricus by using the E. coli pET expression system. HP Heat precipitation at 70°C, IEC ion exchange chromatography, GF gelfiltration, M protein ladder (Page ruler™, fermentas)