Fig. 9.

SDS PAGE gel (a) and western blot (b) showing homologous expression and purification of the S. solfataricus GAD (SSO3198). a Coomassie stained 12.5% PAA gel of His tag-specific affinity chromatography fractions. b Detection of the blotted S. solfataricus GAD using Strep-Tactin, revealing a protein of about 49 kDa (including tandem tag). M Protein standard, CE crude extract, FT flow through, W1-3 washing fractions, E1-3 elution fractions