Table 8.
Kinetic parameters of the GAPN (SSO3194) assayed at 80 and 70°C
| D,L-GAP (mM) | NADP (mM) | Assay temp. (°C) | V max (U/mg) | K m (mM) | K cat (min−1) (s−1) | K cat/K m (mM−1 s−1] |
|---|---|---|---|---|---|---|
| 3 | 2 | 80 | 10.58 | 0.95 | 544.97 9.08 | 9.51 |
| 3 | 2 | 70 | 7.46 | 1.51 | 384.17 6.40 | 4.25 |
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