Fig. 5.

Signaling pathways involved in Zn deficiency-induced apoptotic cell death. IMR-32 cells (left panels) and rat cortical neurons (right panels) were incubated in control non-chelated media (C) or chelated media containing 1.5 (1. 5 Zn) or 15 μM (15 Zn) Zn (for IMR-32 cells) for different periods of time. a Western blots for phosphorylated ERK1/2 (p-ERK1/2), ERK1/2, phosphorylated Akt (p-Akt), and Akt after 9 h incubation. b Western blots for phosphorylated Bad at serine 136 [p-Bad (Ser 136)] or serine 112 [p-Bad (Ser 112)]. After quantitation results are expressed as the ratio phosphorylated/non-phosphorylated protein. All results are shown as means ± SEM of three to four independent experiments. * Significantly different compared to C and 15 μM Zn groups (P < 0.05, one-way ANOVA test)