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. 2009 Dec 10;28(1):150. doi: 10.1186/1756-9966-28-150

Figure 2.

Figure 2

Real-time PCR analysis of upregulated or downregulated gene expression in response to HIF-1alpha (A) Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time PCR. In three pairwise comparisons, the upregulation-folds of IGFBP5, IRS4, TNFAIP6, SOCS1, IL-6, VEGF-A mRNA expression were calculated. The mean and standard error are shown (p < 0.05). (B) Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time PCR. In three pairwise comparisons, the downregulation-folds of IGFBP3, ZNF569, SOCS2, SIRPa and XRCC4 mRNA were calculated. The mean and standard error are shown (p < 0.05).