Table 3.
Average cDNA pool coverages by enzyme combination across 92 eukaryotes.
| Enzyme Combination | Coverage ± SD | Min-Max Coverage | R2 | Regression Equation |
|---|---|---|---|---|
| MseI & CviAII | 76.13 ± 10.51 | 42.07 - 91.97 | 0.94 | Nbp = 1849399*AF+294835 |
| CviAII & CviQI | 72.55 ± 10.32 | 46.44 - 93.68 | 0.98 | Nbp = 1924913*AF+1067365 |
| CviAII & TaqI | 69.56 ± 13.62 | 32.19 - 96.94 | 0.85 | Nbp = 2152800*AF+933639 |
| MseI & CviQI | 66.63 ± 10.32 | 36.63 - 86.15 | 0.94 | Nbp = 2731950*AF-862614 |
| CviAII & MaeII | 64.16 ± 13.93 | 33.34 - 91.88 | 0.91 | Nbp = 2309466*AF+1759142 |
| MaeI & CviAII | 63.20 ± 11.42 | 21.81 - 85.13 | 0.90 | Nbp = 2306663*AF+3307692 |
| MseI & TaqI | 62.69 ± 14.23 | 28.35 - 90.91 | 0.72 | Nbp = 2995858*AF+626737 |
| HpaII & CviAII | 62.00 ± 18.32 | 9.45 - 93.30 | 0.94 | Nbp = 1890564*AF+3666024 |
| TaqI & CviQI | 61.26 ± 14.41 | 25.91 - 94.55 | 0.76 | Nbp = 2726475*AF+2079328 |
| MseI & MaeI | 58.28 ± 12.22 | 27.61 - 79.49 | 0.84 | Nbp = 2852998*AF+2622539 |
| MseI & MaeII | 57.15 ± 12.86 | 29.81 - 84.18 | 0.85 | Nbp = 3630319*AF-565133 |
| MaeII & CviQI | 55.70 ± 15.43 | 23.57 - 88.18 | 0.85 | Nbp = 3156237*AF+1906975 |
| MaeI & CviQI | 54.91 ± 11.08 | 21.14 - 81.68 | 0.91 | Nbp = 3400108*AF+2063017 |
| TaqI & MaeII | 54.86 ± 17.23 | 19.16 - 92.44 | 0.64 | Nbp = 2762584*AF+4754785 |
| HpaII & MseI | 54.39 ± 14.73 | 12.13 - 87.81 | 0.92 | Nbp = 3607697*AF+484514 |
| HpaII & CviQI | 54.14 ± 18.34 | 9.05 - 91.74 | 0.88 | Nbp = 2623528*AF+3657451 |
| HinP1I & CviAII | 53.57 ± 19.96 | 5.08 - 95.05 | 0.85 | Nbp = 2260683*AF+4996193 |
| HpaII & TaqI | 53.54 ± 19.47 | 8.04 - 93.28 | 0.72 | Nbp = 2433331*AF+5040020 |
| MaeI & TaqI | 52.26 ± 13.47 | 17.71 - 85.30 | 0.80 | Nbp = 4183276*AF+518528 |
| HpaII & MaeII | 49.93 ± 19.70 | 5.49 - 89.29 | 0.79 | Nbp = 2690086*AF+4940474 |
| HinP1I & CviQI | 47.88 ± 19.79 | 5.27 - 89.77 | 0.79 | Nbp = 2909507*AF+5271739 |
| MaeI & MaeII | 47.76 ± 12.04 | 15.32 - 78.81 | 0.93 | Nbp = 4923589*AF-278386 |
| HinP1I & TaqI | 47.32 ± 20.70 | 4.26 - 91.43 | 0.63 | Nbp = 2600056*AF+7269281 |
| HinP1I & MseI | 46.22 ± 15.07 | 6.86 - 87.60 | 0.84 | Nbp = 4211767*AF+2594577 |
| HpaII & MaeI | 45.75 ± 15.11 | 4.16 - 80.64 | 0.92 | Nbp = 4048888*AF+3106105 |
| HinP1I & HpaII | 45.05 ± 22.78 | 1.36 - 94.75 | 0.78 | Nbp = 2272295*AF+6925901 |
| HinP1I & MaeII | 44.15 ± 20.69 | 4.08 - 89.75 | 0.71 | Nbp = 2937393*AF+6703632 |
| HinP1I & MaeI | 39.00 ± 15.30 | 2.53 - 77.47 | 0.89 | Nbp = 5159080*AF+3260514 |
Descriptive statistics on the average cDNA pool coverage obtained for each enzyme combination across all 92 species (see additional file 1), sorted by decreasing mean coverage. The average coverage by enzyme combination and standard deviation (Coverage ± SD) are indicated, as are the minimum and maximum cDNA pool coverages for individual enzyme combinations (Min-Max Coverage). R-Square indicates the correlation coefficient for the relationship of total cDNA pool size (Nbp) and the average number of fragments produced per selective PCR (AF). The linear regression equation for this relationship is indicated.