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Gating strategy and flow cytometry sorting analysis of central (TCM) and effector (TEM) memory CD8+ T-cell populations from a rhesus macaque (DBN2) immunized with a DNA vaccine construct containing full length SIVmac239 Gag, Pol, Vif, Tat, and Nef sequences. CD8+ lymphocytes were singlet-gated and defined as memory cells based on surface expression of CD95. TCM and TEM fractions were further defined and sorted based on CD28 and CCR7 expression.
