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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):315–326. doi: 10.4049/jimmunol.0902410

Figure 2.

Figure 2

TCM- and TEM-derived CD8+ T-cell clones exhibit similar functional reactivity and both display an effector memory surface phenotype after culture in vitro. TCM- and TEM-derived SIV-specific CD8+ T-cell clones from two rhesus macaques, AZ15 and DBN2, were stimulated with PBMC pulsed with the appropriate peptides (A) or autologous SIV-infected CD4+ T-cell clones (B). IFN-γ-expression by the TCM- and TEM-derived clones was measured by ICS and flow cytometry. The surface phenotype of TCM- and TEM-derived clones was determined using flourochrome conjugated mAbs to CD28 and CD95 followed by flow cytometric analyzes (C).