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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):315–326. doi: 10.4049/jimmunol.0902410

Figure 6.

Figure 6

Adoptive transfer of virus-specific CD8+ T cells does not significantly affect the endogenous SIV-specific T-cell response. (A) PBMC collected at the indicated time points from the two Mamu A*01 positive monkeys, DBN2 and DBK1 were stained with SIV Tat SL8 or Gag CM9 tetramers and CD8 mAb. (B) PBMC from DBN2 and DBK1 collected pre- and post- infusion were stimulated with SIV Tat SL8, Gag CM9 and Acc peptide pool and the percentage of CD8+ IFN-γ-producing T cells determined by flow cytometry, or (C) the number of IFN-γ-producing cells determined by ELISpot. (D) PBMC from AZ15, DBN2 and DBK1 collected on day 39 post infusion were stimulated with SIV Acc, Env, Gag and Pol peptide pools and the number of antigen-induced IFN-γ-producing T cells determined by ELISpot.