Table I.
CD8+ T cell specificities, MHC class I restriction and number of cells infused
| Monkey ID (Mamu) | CD8+ T cell specificity (origin)a | Mamu restriction | bNumber of cells infused |
|---|---|---|---|
| AZ15 (A*02) | Nef-YY9-7 (TEM) | A*02 | 0.8×109 |
| Nef-YY9-12 (TEM) | A*02 | 1.4×109 | |
| Vif-WY8-10 (TCM) | A*02 | 1.6×109 | |
| DBN2 (A*01/*02) | Gag-CM9-18c (TEM) | A*01 | 5.8×109 |
| Gag-CM9-19 (TCM) | A*01 | 2.6×109 | |
| Vif-WY8-4 (TCM) | A*02 | 2.1×109 | |
| Vif-WY8-39 (TEM) | A*02 | 1.6×109 | |
| DBK1 (A*01) | No CD8+ T cells | N/A | N/A |
a
CD8+ T cells from AZ15 and DBN2 were sorted based on surface phenotype (central memory T cells, TCM: CD28+CD95+CCR7+; effector memory T cells, TEM: CD28− CD95+CCR7−) before generation of SIV-specific lines, cloning and characterization in vitro.
b
Total number of cells of each clone included in pool of cells infused intravenously; half of the cells from each clone were stained with either PKH26 (TCM) or CFSE (TEM). N/A, not applicable.