Table III.
Mutations in CD8+ T cell epitope, SIV Nef YY9, following adoptive transfer
| Monkey ID |
Peptide sequencec |
Sample dated |
% IFN-γ-producing cellse |
|---|---|---|---|
| AZ15 a(A*02) | Nef159-167YY9b YTSGPGIRY (wild type) | 74 | |
| YSSGPGIRY (T2S variant) | 9-17 (12-20) | 44 | |
| |
YSSGPGIRF (T2S; Y9F variant) |
35-60 (38-63) |
4 |
| DBN2 a(A*01/*02) | YTSGPGIRF (Y9F variant) | 35-60 (38-63) | 27 |
a
Monkey MHC class I (Mamu) type of interest.
b
Mamu A*02-restricted epitope.
c
Wild type epitope sequence and observed mutations in virus isolated from acutely infected rhesus macaques; amino acid replacements in variant peptides underlined in bold.
d
Time frame in days post infusion (infection) when mutation was observed i.e. mutation not seen at lower time point in range but seen at higher time point.
e
Frequency of cells positive for IFN-γ in clonal population of CD8+ T cells generated against WT peptide after stimulation with APC pulsed with 2 μg/mL of WT or variant peptide; analyses by ICS and flow cytometry.