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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Anesthesiology. 2009 Oct;111(4):741–752. doi: 10.1097/ALN.0b013e3181b27fd4

Figure 3. Treatment of nitrous oxide plus isoflurane induces caspase-3 activation and apoptosis in primary neurons from naïve mice.

Figure 3

Figure 3

Figure 3

A. Effects of 70% nitrous oxide plus 1% isoflurane for six hours, and staurosporine on caspase-3 activation. Each band in the Western blot represents an independent experiment. B. Quantification of the Western blot, normalized to β-actin levels. We have averaged results from three independent experiments. C. Effects of the nitrous oxide plus isoflurane treatment, and staurosporine treatment on TUNEL positive cells. We have averaged results from four independent experiments. TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; FL, full length. * P < 0.05; ** P <0.01; ## P < 0.01.