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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Anesthesiology. 2009 Oct;111(4):741–752. doi: 10.1097/ALN.0b013e3181b27fd4

Figure 4. Nitrous oxide treatment alone induces neither apoptosis nor increases in levels of BACE and Aβ in H4-APP cells.

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

A. Effects of 70% nitrous oxide on caspase-3 activation. Each band in the Western blot represents an independent experiment. B. Quantification of Western blot, normalized to β-actin levels. We have averaged results from three independent experiments. C. Effects of the nitrous oxide treatment on apoptosis. We have averaged results from six independent experiments. D. Effects of the nitrous oxide treatment on BACE levels. Each band in the Western blot represents an independent experiment. E. Quantification of the Western blot, normalized to β-actin levels. We have averaged results from three independent experiments. F. Effects of the nitrous oxide treatment on Aβ levels. We have averaged results from six independent experiments. BACE, β-site amyloid precursor protein-cleaving enzyme; APP, amyloid precursor protein; Aβ, β-amyloid protein; FL, full length. N.S., not significant; ** P <0.01