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. Author manuscript; available in PMC: 2010 Dec 29.
Published in final edited form as: Biochemistry. 2009 Dec 29;48(51):12242–12251. doi: 10.1021/bi901489n

Figure 3. Observed Rates for NADH Binding to Native PGDH in the Presence of Satutrating Levels of L-Serine.

Figure 3

The observed rates (kobs) derived from the pre-steady state transients are plotted versus NADH concentration. kobs 1(●), kobs 2 (■), kobs 3 (○), kobs 4 (▲). The solid lines are fits of the data to equation 2. The protein concentration was 2.5 μM subunit.