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Spectral changes during reduction of PAO by N,N'-bis(4-CH3-benzyl)-1,4-diaminobutane at pH 6.6, 30 °C: (A) changes at 458 nm (only 1/30th of the points are shown for clarity); (B) changes in the entire visible absorbance spectrum; (C) initial (—) and final (…) spectra from a global analysis of the data in B using a single-step irreversible reaction as the model. The line in A is from a fit of the data to eq 4.
