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Effect of pH on the D(kcat/Km) and Dkcat values for para-substituted N,N'-dibenzyl-1,4-diaminobutanes as substrates for PAO
| pH | Kinetic Isotope Effects | ||
|---|---|---|---|
| X = H | X = CH3O | X = CF3 | |
| 7.1 | 1.3 ± 0.1 | 1.7 ± 0.1 | n.d. |
| 7.6 | 1.7 ± 0.1 | 1.8 ± 0.1 | 1.4 ± 0.1 |
| 8.1 | 1.8 ± 0.1 | 1.7 ± 0.1 | 1.5 ± 0.1 |
| 8.6 | 2.0 ± 0.1 | 1.7 ± 0.1 | 1.6 ± 0.1 |
| 9.1 | 2.1 ± 0.2 | 1.7 ± 0.1 | 1.5 ± 0.1 |
| 9.6 | 2.0 ± 0.2 | 1.6 ± 0.1 | 1.6 ± 0.1 |
| 10.1 | 1.9 ± 0.1 | 1.6 ± 0.1 | 1.6 ± 0.1 |
| Average | 1.8 ± 0.3 | 1.7 ± 0.1 | 1.5 ± 0.1 |
n.d. not determined
