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Individual HIV-1gp120, V2-, and V3- specific IFN-γ (a), IL-2 (b) and IL-4 (c) cytokine-secreting cells per 4 × 105 PBMC (26). Controls (n = 2 FluISCOM plus n = 2 wtFP) grouped together with HIV-1 ISCOM and HIV-1FP groups, respectively. The y axis for each cytokine is used based on the optimal working range for each cytokine. IFN-γ (0 -100), IL-2 (0–15), and IL-4 (0–100), respectively.
