Table 1.
Individual immune responses immediately before challenge and virus status post challenge
| Animal vaccine | Immune responses prechallenge*
|
Postchallenge virology†
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| VNT‡
|
gp120‡ | LP§
|
CTL¶
|
PCR (vi) (week)
|
|||||
| SF2 | SF13 | titers | SI (cpm) | Pep. pools (% lysis) | 2 | 4 | 6 | Status | |
| HIV-I ISCOM | |||||||||
| 9263 | 400 | 320 | 2,500 | 4.1 (5554) | – | −(0) | −(0) | −(0) | protected |
| BB85 | 200 | 160 | 2,500 | 4.3 (441) | 41–52 (16.4%) and V2, V3 (19%) | −(0) | −(0) | −(0) | protected |
| 9251 | 200 | 160 | 2,500 | 2.8 (1985) | 1–12 (8.0%) and 13–24 (10.4%) | −(0) | −(0) | −(0) | protected |
| 9111 | 400 | 160 | 2,500 | 2.6 (436) | – | −(0) | −(0) | −(0) | protected |
| Controls | |||||||||
| 9258 | – | – | 0.8 (196) | – | +(270) | +(270) | +(25) | infected | |
| BB70 | – | – | 1.3 (144) | – | +(90) | +(30) | +(12.5) | infected | |
| BB102 | – | – | 1.0 (608) | – | +(90) | +(270) | +(12.5) | infected | |
| 9219 | – | – | 0.6 (235) | – | +(810) | +(810) | +(50) | infected | |
| HIV-1 fowlpox | |||||||||
| 4044 | >160 | 20 | 500 | 1.8 (548) | – | +(30) | +(90) | +(16.7) | infected |
| 9264 | >80 | 20 | 100 | 0.9 (172) | – | +(30) | +(10) | +(1.0) | infected |
| BB98 | – | – | – | 1.2 (170) | – | +(90) | +(90) | +(25) | infected |
| BB108 | 160 | – | 100 | 5.4 (689) | – | +(270) | +(30) | +(25) | infected |
| Statistics | |||||||||
| Kruskal Wallis‖ | 5.69–7.54 | n.a. | 8.77 | 6.04 (3.04) | 8.16 | 8.54 | 7.42 | ||
| P value | <0.049 | n.a. | <0.008 | 0.049 > P > 0.011 (> 0.104) | <0.008 | <0.008 | 0.049 > P > 0.011 | ||
| ISCOMS VS FOWLPOX: | |||||||||
| Wilcoxon** | >1 | 0 | 0 | 2 (−) | 0 | 0 | 0 | ||
| P value | NS | <0.05 | <0.05 | NS (−) | <0.05 | <0.05 | <0.05 | ||
SI, stimulation index; NS, not significant; n.a., not applicable.
Immune responses are from samples taken two weeks after the third immunization.
Post challenge virology was based on DNA-PCR (28) as represented as positive (+) or negative (−), and quantitative virus isolation (vi) given as the number of virus producing cells in 1 × 107 PBMC (36, 37). Animals negative by both assays after more than five consecutive samplings were classified as protected.
LP given as stimulation index, SI or cpm.
CTL responses performed as described (14); CTL based on >10% specific lysis at two or more E:T ratios (specific lysis is shown at an E:T ratio of 5:1) on autologous peptide pulsed target cells are represented by the letter of the peptide pools (overlapping 15 mers) giving positive responses.
Statistical analysis to determine if there was a significant difference between the three groups.
To determine if there was statistical significance between the two HIV-1 vaccine groups.