Figure 2. The FHA domain of FipA is required for its interaction with FtsZ.
A. Cell lysates of M. tuberculosis or M. smegmatis were immunoprecipitated (IP) with anti-FtsZ antibody and immunoblotted (IB) with anti-FipA antibody or antibody against an irrelevant protein (PPK1) [as a negative control]. The blot was reprobed with anti-FtsZ antibody to show equal loading. B. Lysates of M. smegmatis mc2155 or FipA-KO complemented with the wild type (WT) or different mutants of FipAMTB, were immunoprecipitated with anti-FipA antibody followed by immunoblotting with anti-FtsZ antibody and reprobing with anti-FipA antibody. Blots shown are representative of three separate experiments.