Table 1. Distribution of T cells subsets based on CD3, CD4, CD8 amongst melanoma patients PBMC and tumor associated lymphocytes and healthy donor PBMC.

	FREQUENCY
Cell origin	CD3+	CD4+	CD8+	CD4+CD8+
PBMC
healthy donors (n = 11)	70,5+/−6,3	46+/−10	9.5+/−9	0.9+/−0.6
melanoma patients (n = 6)	68,5+/−7,1	51.1+/−6	14.9+/−3	0.6+/−0.2
Melanoma Associated Lymphocytes
ILNL (n = 30)	99,9+/−0,2*	31.1+/−26	54.2+/−25***	4.2+/−5.6
TIL (n = 10)	98,4+/−9,1*	35,3+/−26	50,3+/−26**	9,5+/−8,3**

Results are expressed as median fraction of cells expressing the marker(s) +/− SD among total cells. Significant differences were evaluated by comparison with similar cell fractions among melanoma cancer patient PBMC using the Tukey-Kramer's test. *P<0.05, **P<0.01, ***P<0.001.