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. 2010 Jan 15;6(1):e1000809. doi: 10.1371/journal.pgen.1000809

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Michos et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A,B) In situ hybridization in transverse sections of E10.5 wild type embryos reveals that Fgf10 and Gdnf are expressed in metanephric mesenchyme (arrows). (C,D) Whole-mount in situ hybridization at E11.0 (dorsal view) shows that Fgf10 and Gdnf are expressed in metanephric mesenchyme (MM) surrounding the UB epithelium. The schematic diagram illustrates Fgf10 expression, with purple indicating where the hybridization signal was detected. (E–G) Visualization of Hoxb7/myrVenus shows (E) normal UB branching in an Fgf10+/− kidney, (F) reduced branching in an Fgf10−/− kidney, and (G) rescue of UB branching in an Fgf10−/− kidney when Spry1 dosage is reduced (Spry1+/−). Scale bars, 100 µm. (H–J) Induction of ectopic budding from the Wolffian duct by FGF10. Dissected E10.5 urogenital regions were cultured with control PBS-soaked beads (H) or beads soaked in FGF10 (I,J) placed between the two Wolffian ducts (dotted yellow circles). FGF10 induces multiple ectopic UB outgrowths (marked by asterisks) in both control Gdnf+/− (I) and Gdnf−/− (J) samples. Open arrowhead in H, Wolffian duct; arrows in H-I, normal ureteric buds.