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. 2010 Jan 7;5(1):e8627. doi: 10.1371/journal.pone.0008627

Figure 1. Expression of HCN mRNA.

Figure 1

(A) Conventional RT-PCR was used to examine the expression of HCN1-4 in vestibular epithelia of wild-type mice. Arrows indicate the expected size of the PCR product: HCN1: 491 bp; HCN2: 337 bp; HCN3: 339 bp and HCN4: 419 bp. Lane 1 in each gel contained markers. Each subsequent lane contained the PCR product obtained using template cDNA harvested from the following sources. Lane 2: wild-type mouse brain; Lane 3: HCN1-deficient mouse brain; Lane 4: wild-type utricle; Lane 5: HCN1-deficient mouse utricle. (B) Quantitative RT-PCR was used to estimate total number of copies of messenger RNA for each of the four HCN subunits using wild-type mouse cochlea as template. Total copies in thousands of HCN mRNA transcripts per cochlea. Results were from a pool of 4 cochlea obtained from mice at P4.