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Validation of differential expression in islets by quantitative real-time PCR. TaqMan quantitative PCR validation of genes differentially regulated between CHF (white bars) and HF (black bars). The change in expression is shown for selected genes involved in islet growth and development, protein processing and secretion, metabolism, and signalling. Values are normalised to Actb. Data are mean ± SE of five separate mice per group. *p ≤ 0.05
