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HDX-MS -based construct optimization of VPA0419 protein from V. parahaemolyticus (NESG target, VpR68). a 1H-15N HSQC spectra (20°C) of full length (res. 1-109) and b construct optimized (res. 17-99) solved by NMR (PDB ID: 2JZ5). c HDX-MS results for full-length of V. parahaemolyticus VPA0419 (10, 100, and 1,000 s 1H/2H exchange durations), the legend indicates the color-coded H/D exchange rate
