Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jan 8;5(1):e8619. doi: 10.1371/journal.pone.0008619

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

(A) Outer membrane vesicles (800 µg protein) were solubilised with 0.75% (w/v) dodecyl-maltoside and the BAM complex immunoprecipitated with an antiserum recognizing the BamA subunit (lane 3) or preimmune serum (lane 4). Samples corresponding to 8 µg protein of total outer membranes (Lane 1), 8 µg protein of unbound fraction (Lane 2) and all of the material immunoprecipitated (Lane 3, 4) are shown. Asterisks indicate the IgG heavy and light chains, with the migration positions of the molecular markers shown in kDa. (B) Mass spectrometry data summarizing the identification of immunoprecipitated proteins (see Methods). In addition to the number of high confidence peptides identified by MS/MS data and sequence coverage; a MOWSE score is included, which is a probabilistic score that indicates the match of the experimental peptide precursor masses (peptide mass fingerprint) to the sequence of a candidate parental protein. Typically a MOWSE score >75 is considered as significant [64].