Figure 4. ACAP-A/B are involved in spore generation but not germination.

48 h after development on nutrient deficient agar, spores from wild-type, two independent clones of acap-A⁻/B ⁻ and one off-target insertion clone (Fig. 2B, clone 7) were collected and treated without or with 0.5% SDS as described under “Methods.” A and B, a fixed volume of spore suspension was plated on bacterial lawns. The number of germinating AX2 spores was 269±95 per µl after treated with SDS (B), which was about 89% of the germinating spores without SDS treatment (A). C, spore concentrations were counted and 30 spores were plated on bacterial lawns. The number of plaques formed from each sample was scored and expressed as a percentage relative to plaques formed by wild-type spores. Data shown were the mean±s.e.m. of at least three independent experiments. * indicates significantly different from wild-type by one way ANOVA using Dunnett's multiple comparison test, p<0.05. D, AX2 and acap-A⁻/B ⁻ fruiting bodies were isolated and spores in the sori were imaged by DIC microscopy. E, individual spores from AX2, off-target insertion and acap-A⁻/B ⁻ cells were harvested, treated as in (A) and (B), and viewed by DIC microscopy. Bars, 10 µm (D); 4 µm (E).