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. 2010 Jan 7;5(1):e8615. doi: 10.1371/journal.pone.0008615

Figure 2. Validation of P gene siRNA using pAcGFP1N1-CHPV-P.

Figure 2

(a) Fluorescent microscopic images of HEK-293 cells transfected with different P gene siRNAs and plasmid pAcGFP1N1-CHPV-P. (b) P gene siRNAs validated by flowcytometry at the protein level (c) Reduced expression of P gene mRNA by real time one step RT-PCR. (d) P gene validation with CHPV at different time points. (e) Effect of different doses of P-2 siRNA on the expression of P gene as determined by flowcytometry and real time one step RT-PCR. Result are expressed as means ± SD. * indicates significantly different from control at p <0.05 by Dunnett's test.