Table 2. Mutant siRNAs used for determining tolerance of mismatches in the P-2 siRNA.
S. No | Name | Nucleotide | Target sequence (Change is shown in Bold) |
1. | P-2 (7) | (A --T ) | CGAATCTCCTGGCTCCAAA |
2. | P-2 (8) | (C--G ) | CGAATCAGCTGGCTCCAAA |
3. | P-2 (9) | (C--G ) | CGAATCACGTGGCTCCAAA |
4. | P-2 (10) | (T--A ) | CGAATCACCAGGCTCCAAA |
5. | P-2 (7–8) | (AC-TG ) | CGAATCTGCTGGCTCCAAA |
6. | P-2 (9–10) | (CT-GA ) | CGAATCACGAGGCTCCAAA |
7. | P-2 (7–10) | (ACCT-GTTC ) | CGAATCGTTCGGCTCCAAA |