Table 3.

Mutation Frequency in Correlation with Tumor Cell Content

	% of tumor cells in tissue
	Total	≤10%	>10%	P value
Sanger sequencing*				0.015
Mutated	108 (41.9%)	0 (0%)	108 (43.2%)
Not mutated	150 (58.1%)	8 (100%)	142 (56.8%)
Array				0.101
Mutated	104 (44.6%)	1 (14.3%)	103 (45.6%)
Not mutated	129 (55.4%)	6 (85.7%)	123 (54.4%)
Melting curve				0.059
Mutated	77 (41%)	0 (0%)	77 (42.1%)
Not mutated	111 (59%)	5 (100%)	106 (57.9%)
Pyrosequencing				0.175
Mutated	51 (37.5%)	0 (0%)	51 (38.3%)
Not mutated	85 (62.5%)	3 (100%)	82 (61.7%)

P values were calculated by χ² test.

^*In two cases estimation of exact tumor cell content was not possible.