Examples of aberrant DHPLC profiles. The figure shows different DHPLC profiles with growing complexity from A to D. A: Exon 6 showed a heteroduplex in both pools 1 and 4 sharing the DNA sample TU19, in which a frameshift mutation (c.401 404 delCCAA) was detected. B: Exon 27 heteroduplexes in both pools 2 and 6 sharing the DNA sample TU124, in which a splicing mutation (c.3433-1 A>G) was detected. C: Exon 29 heteroduplexes in pools 1, 4, and 5. Pools 1 and 4 shared the DNA sample TU181, pools 1 and 5 shared the DNA sample TU188. The same nonsense mutation (c.3940 C>T) was detected in both samples. D: Three different exon 14 heteroduplexes in pools 2 and 6 and 1 and 4, corresponding to combination of a mutation (**) and a known polymorphism (*). Arrow indicates homoduplexes.