Table 2.

Comparison of Mutation Detection Methods in Clinical Samples

Case	SmartAmp2	Direct seq	Enzyme-enriched	PNA-enriched	AA change
1	Mut	Wt	Wt	G35T	G12V
2	Mut	Wt	G34T	G34T	G12C
3	Mut	Wt	G35A	G35A	G12D
4	Mut	G35T	G35T	G35T	G12V
5	Mut	Wt	G35A	G35A	G12D
6	Mut	Wt	G35C	G35C	G12A
7	Mut	G35T	G35T	G35T	G12V
8	Mut	Wt	Wt	Wt	G12D
9	Mut	G34T	G34T	G34T	G12C
10	Mut	Wt	Wt	Wt	G12C
11	Mut	G34T, G35T	Wt	G34T, G35T	G12F
12	Mut	G35T	G35T	G35T	G12V
13	Mut	G35T	G35T	G35T	G12V
14	Mut	G35C	G35C	G35C	G12A
15	Mut	G35C	G35C	G35C	G12A
16	Mut	G35A	G35A	G35A	G12D
17	Mut	G35A	G35A	G35A	G12D
18	Mut	Wt	G35A	G35A	G12D
19	Mut	G35A	G35A	G35A	G12D
20	Mut	G35T	G35T	G35T	G12V
21	Mut	Wt	G34T	G34T	G12C
22	Mut	Wt	G34T	G34T	G12C
23	Mut	Wt	G35A	G35A	G12D
24	Mut	G35A	G35A	G35A	G12D
25	Mut	G35A	G35A	G35A	G12D
26	Mut	Wt	Wt	Wt	G12C
27	Mut	Wt	G35A	G35A	G12D
28	Mut	G35A	G35A	G35A	G12D
29	Mut	G35A	G35A	G35A	G12D
30	Mut	Wt	G35A	G35A	G12D
31	Mut	Wt	G34T	G34T	G12C
Mut rate (n = 172)	18.0%	9.3%	15.1%	16.3%

Direct seq, PCR-based direct sequencing; enzyme-enriched, enzyme-enriched sequencing; PNA-enriched, PNA-enriched sequencing; AA change, amino acid change; Mut, mutant allele; Wt, wild-type allele.