Figure 3.

Amino acid sequences of AMACR-derived peptides and their HLA-A24 binding assay. A. Amino acid sequences of AMACR protein and four peptides (AMACR1-4) with HLA-A24 binding motif (underlines). The predicted anchor residues to HLA-A24 are indicated in boldface within the amino acid sequences of the peptides. B Binding affinities of AMACR-derived peptides to HLA-A24 molecule were evaluated by the mean fluorescence intensity (MFI) of cell surface HLA-A24 molecules on T2-A*2402 cells that were pulsed with each peptide. EBV LMP2-derived peptide (TYGPVFMSL) and HIV env-derived peptide (RYLRDQQLLGI) were used as positive controls for HLA-A24-bound peptides. SL-8 peptide (SIINFEKL) was used as a negative control.