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. 2009 Dec 9;7:103. doi: 10.1186/1479-5876-7-103

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Copyright ©2009 Honma et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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AMACR1 peptide-specific CTL induction from PBMCs of HLA-A24-positive prostate cancer patients. PBMCs of HLA-A24-positive prostate cancer patients (cases 4, 5 and 6) were stimulated four times with three kinds of AMACR peptide (AMACR1-3)-pulsed APCs and their cytotoxic activities were examined by ⁵¹Cr release assay at the indicated effector/target ratios. AMACR1 peptide-pulsed T2-A*2402 cells served as target cells. Non-pulsed T2-A*2402 cells were used as negative control target cells. K562 target cells were used for monitoring natural killer cell activity and lymphokine-activated nonspecific cytotoxicity.